2 edition of Studies into the production of P22 generalised transducing particles in Salmonella typhimurium. found in the catalog.
Studies into the production of P22 generalised transducing particles in Salmonella typhimurium.
Timothy William Brown
Thesis (Ph.D.) - Univ. of Birmingham, Dept of Genetics.
Genetic transduction by phages and chromosomal islands: The new and noncanonical Yin Ning Chiang ID 1, times encapsidate host bacterial DNA to form transducing particles. Transducing particles are Salmonella typhimurium with Pmutants with increased transducing ability (HT Cited by: 2. Feb 01, · Generalized transducing bacteriophages are valuable members of the arsenal of tools for the genetic study of bacteria. ES18 is a temperate, generalized transducing double-stranded DNA (dsDNA) phage that naturally infects Salmonella enterica serovar Typhimurium (), as well as some serovar Enteriditis, Dublin, Pullorum, Gallinarum, and Paratyphi B strains (52, 76).Cited by:
Start studying test 3. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Search. Salmonella typhimurium P what is the headfull rule. Specialized transducing particles. Have both host and phage DNA. ( regions of each) GTP or STP, which conserves a . Generalized transduction involves the packaging of random host DNA (genomic or plasmid) into a bacteriophage particle and the transfer of that DNA to a recipient strain. Bacteriophage P22 is a generalized transducing phage that is commonly used for genetic experiments with Salmonella (Zinder and Lederberg, ; Kropinski et al., ).Cited by:
The obtained relationships depend significantly on the number of classes of transducing fragments. The model was applied to estimate the number of transducing fragment classes for a given marker in transduction with phage P22 of Salmonella typhimurium. It was found that the literature data on frequencies of cotransduction in crosses with mutual substitution of selective and nonselective Cited by: 1. A random insertion transposon library of , mutants in Salmonella enterica serovar Typhimurium was used to monitor effects of individual bacterial gene disruptions on bacteriophage P22 lytic infection. These experiments revealed candidate host genes that alter the timing of phage P22 senhoras.site by: 2.
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The temperate bacteriophage P22 mediates both generalized and specialized transduction in Salmonella typhimurium. Specialized transduction by phage P22 is different from, and less restricted than, the well characterized specialized transduction by phage lambda, due to differences in the phage DNA packaging senhoras.site by: 9.
The generalized transducing particles are the same size as P22 phage particles but instead of phage DNA they contain bacterial DNA which was synthesized before phage infection.
Further, DNA molecules isolated from the transducing particles have the same molecular weight as P22 DNA Cited by: The temperate bacteriophage P22 mediates both generalized and specialized transduction in Salmonella typhimurium.
Specialized transduction by phage P22 is different from, and less restricted than, the well characterized specialized transduction by phage lambda, due to differences in the phage DNA packaging senhoras.site by: 5. later demonstrated to be identical to the bacteriophage P InLennox () reported that, similarly, the temperate coliphage P1 could also act as a vector to carry out what has come to be termed generalized transduction.
Transducing bacteriophage particles are formed in donor bacterial cells during phage development. They. Dec 01, · CsCl density gradient analysis showed that the DNA of plaque forming particles of Salmonella phage P22is lighter than the host DNA.
The DNA of transducing phages exhibits an intermediate density, Cited by: The generalized transducing phage P22 of Salmonella typhimurium was involved in the initial discovery of transduction by Zinder and Lederberg in P22 is a member of the Podoviridae, with a 57 nm icosahedral head, a short tail, a sort of baseplate made up of six trimers of the tailspike protein, and a single fiber extending from the middle of the baseplate.
Bezdek, M., Amati, P.: Properties of P22 and a related Salmonella typhimurium phage. General features and host specificity. Botstein, D.: Superinfection exclusion by P22 prophage in lysogens of Salmonella typhimurium.
Exclusion of generalized transducing particles. The production of inactive phage P22 particles following inductionCited by: VIROLOGY 27, () Heterogeneity in P22 Transducing Particles JOHN R. ROTH AND PHILIP E.
HARTMAN Department of Biology, The Johns Hopkins University, Baltimore, Maryland Accepted July 32, A gene, hisR, involved in the regulation of levels of histidine biosynthetic en- zymes in Salmonella typhimwium, maps between a gene, metE, involved in methio- nine Cited by: Most genetic analysis in Salmonella is performed using Pmediated transductional crosses.
Salmonella is an attractive genetic system largely because its phage (P22) is easy to propagate, stable during storage, and an efficient transducer. Mutational modification of phage P22 has improved its usefulness by increasing its transducing frequency and preventing stable lysogeny so that phage.
Characterization of SE1, a New General Transducing Phage of Salmonella typhimurium Article (PDF Available) in Journal of general microbiology (4) · May with 36 Reads. Phage Pmutants with increased or decreased ability to produce transducing particles (HT-1 and NT-mutants) were submitted to mapping experiments.
The gene responsible for HT-phenotype was found to be allelic to gene 3 of the P22 linkage senhoras.site by: The kinetics of production of transducing particles for different bacterial markers were followed by premature lysis of Salmonella typhimurium cells infected with P22 phages.
They were compared for cells infected with wild type phage or with HT-mutants which show increased transduction senhoras.site by: A Novel P22 Prophage in Salmonella typhimurium. ized transducing bacteriophage P22 mutant (HT /1, Cultures were diluted fold into fresh medium and incu.
Transfer of chimeric plasmids among Salmonella typhimurium strains by P22 transduction Article (PDF Available) in Journal of Bacteriology (3) · April with 16 Reads. The generalized transducing bacteriophage of Salmonella typhimurium, P22, can transduce plasmids in addition to chromosomal markers.
Previous studies have concentrated on. Ebel-Tsipis, J. and D. Botstein, Super-infection exclusion by P22 prophage in lysogens of Salmonella typhimurium.
Exclusion of generalized transducing particles. Virology – PubMed Google Scholar. A high-frequency-transducing (HFT) lysate for tetracycline resistance (tetR) was obtained by inducing an unusual tetR P22 lysogen which had been made by transducing Salmonclla typhimurium LT-2 to tetR with P22 phage grown on an LT-2 strain carrying the R factor The HFT lystate contains defective P22 particles, called P22 Tc, Cited by: Discovery of transduction.
InJoshua Lederberg and Norton Zinder were testing for recombination in the bacterium Salmonella typhimurium by using the techniques that had been successful with E. coli. The researchers used two different strains: one was phe− trp− tyr−, and the other was met− his−.
As wild‐type P22 infection produces about 2% generalized transducing particles (Ebel‐Tsipis et al., ), the size of the pac site and specificity of the gene 3 protein binding appear to have evolved to be just stringent enough to ensure that a large fraction of virus‐encoded virion proteins are not spent uselessly (from the phage's.
Transduction with Integration-Defective Mutants of Salmonella typhimurium Bacteriophage KB1 Article (PDF Available) in Journal of Bacteriology (2) · March with 9 Reads. Generalized transduction, discovered in Salmonella phage P22, was the first mechanism of phage-mediated gene transfer to be identified.
It is the process by which phages can package any bacterial DNA (chromosomal or plasmid) and transfer it to another senhoras.site by: 2.E-Mail Address. Password. Forgotten Password? Remember Me.The generalized transducing double-stranded DNA bacteriophage ES18 has an icosahedral head and a long noncontractile tail, and it infects both rough and smooth Salmonella enterica strains.
We report here the complete 46,bp genome nucleotide sequence and provide an analysis of the senhoras.site by: